Saturday 1 July

1700 - 1900  Registration at Artisten
1600 - 1900  Poster mounting at Artisten
1900 - 1910  Welcome note and practical information - S. Hohmann
1915 - 2000  Keynote lecture by P. Agre (Chair: S. Nielsen and S. Hohmann)

2000 - 2130  Welcome reception at Börsen (City Hall), Gustav Adolf Torg, Göteborg.
Bus transportation will be provided from Artisten at 2000

Sunday 2 July

First session: Structure-function analysis

Chair: A. Engel, J. Rydström, and J. Fischbarg

0830 - 0900  A. Engel (key lecture) - Membrane channels for water and small anionic solutes
0900 - 0925  H. Stahlberg - The projection structure of the glycerol channel GlpF at 3.5 Å
0925 - 0950  Y. Fujiyoshi - Novel fold of AQP1
0950 - 1015  A. Mitra - Three-dimensional fold of the human AQP1 water channel determined at 4Å resolution by electron crystallography of 2-dimensional crystals embedded in ice

  1015 - 1045   Coffee

1045 - 1100  W. Skach - Topolological reorientation of aquaporin-1 topology during maturation in the endoplasmic reticulum
1100 - 1115  P. Pohl - Volume flux across red cell Aqp1 and E. coli AqpZ water channel proteins reconstituted into planar lipid bilayers
1115 - 1130  P. Harvengt - Secondary structure, oligomerisation and phosphorylation of lentil seed aquaporins
1130 - 1145  L. Duchesne - Substitution of loops E or/and C-terminal transmembrane domains in aquaporin and glycerol facilitator: Effect on oligomeric state in non denaturing detergents
1145 - 1200  R. Bill - Functional analysis of MIP channels in yeast

1200 - 1400  Lunch and poster viewing
1230 - 1310  authors should be present (posters with even numbers)
1310 - 1355  authors should be present (posters with uneven numbers)

Second session: Physiology and pathophysiology of water transport

Chair: M.A. Knepper, T.E.N. Jonassen and M. Zeidel

1400 - 1440  M.A. Knepper (key lecture) - Physiological roles of aquaporin water channels in the kidney
1440 - 1505  T. Zeuthen - Water transport by cotransport
1505 - 1520  W. Hill - How to construct a water-tight membrane: The apical membrane of MDCK cells as role model
1520 - 1535  A. Masuyk - Microperfused rat intrahepathic bile duct units - a novel functional approach to study water, solute and ion transport in biliary epithelia

1535 - 1605  Coffee

1605 - 1620  T. Matsuzaki - Water channel protein, aquaporin 3, in epithelial cells
1620 - 1635  Y. Ishikawa - Neurotransmitters regulate the amount of AQP5 in the apical plasma membrane via [Ca2+]i in parotoid acinar cells
1635 - 1650  M. Amiry-Moghaddam - Molecular mechanisms of water hemostasis in brain: Are AQP4 molecules subjected to regulation?
1650 - 1715  M. Yasui - Rapid gating of AQP6, an intracellular water channel with anion conductance
1715 - 1730  S. Ishikawa - Upregulation of aquaporin-2 (AQP-2) gene expression in pathological states of water retention in glucocorticoid-deficient (gd) rats
1730 - 1745  E. Baggaley - Prostaglandins and the dehydration-induced upregulation of AQP2 expression
1745 - 1810  C. Ecelbarger - Regulation of renal aquaporins and sodium transporters during vasopressin - escape in the rat

Monday 3 July

Third session: Genetic analysis of aquaporin function

Chair: A.S.Verkman, C. van Os, and A. Menon

0830 - 0910  A. Verkman (key lecture) - Aquaporin physiology revealed by phenotype analysis of transgenic mice
0910 - 0940  J. Schnermann - Requirement of AQP1 for isotonic fluid transport in the renal proximal tubule
0940 - 1005  T. Ma - GI and skin physiology in aquaporin knockout mice

1005 - 1035  Coffee break

1035 - 1100  G. Manley - Aquaporins, cerebral edema, and brain water balance
1100 - 1120  C. Krane - AQP5 deficient (-/-) acinar cells have decreased membrane permeability and altered cell volume regulation

1120 - 1400  Poster viewing and Lunch
1130 - 1215  authors should be present (posters with even numbers)
1310 - 1355  authors should be present (posters with uneven numbers)

Fourth session: Plant systems

Chair: M. Chrispeels, C. Larsson and A.-S. Sandelius

1400 - 1420  M. Chrispeels (key lecture) - The role of aquaporins in cell-to-cell water flow in plant tissues
1420 - 1445  C. Maurel - Physiological and genetic analysis of plasma membrane aquaporin functions in Arabidopsis
1445 - 1510  P. Kjellbom - Posttranslational regulation of aquaporins
1510 - 1525  G. Amodeo - Protoplasmic pH regulates an aquaporin water pathway in plant vacuoles

1525 - 1555  Coffee break

1555 - 1610  M. Carvajal - The response of plants to salinity involve root water channels
1610 - 1625  A. Maggio - Regulation of water channel activity and stomatal conductance are coordinated events in honey locust
1625 - 1640  T. Henzler - Passage of H₂O₂ through water channels: coupling with metabolism and effect of OH radicals
1640 - 1655  R. Kaldenhoff - Expression, location and function of a plasma membrane aquaporin from Tobacco
1655 - 1720  S. Tyerman - Ammonia and water permeation across the peribacteroid membrane of legumes
1720 - 1735  A. Weig - Role of aquaporins during elongation growth of castor bean seedlings

Topical presentation

Chair: P. Agre, J. Frøkiær and S. Nielsen

1735 - 1800  M. Henkemeyer - Bidirectional tyrosine kinase signaling: From ephrins and Eph receptors to aquaporins and anion exchangers

Tuesday 4 July

Fifth session: Aquaporins and glycerol facilitators in microorganisms

Chair: S. Hohmann and B. Prior

0830 - 0900  S. Hohmann (key lecture) - Microbial MIP channels
0900 - 0925  G. Calamita - Biological properties of the microbial Escherichia coli Aquaporin-Z water channel
0925 - 0950  D. Thomas - Study of fast water movements in bacteria by cryoelectron microscopy
0950 - 1005  M. C. Rodriguez - Molecular cloning and characterization of AqpX a water channel from Brucella abortus

1005 - 1035   Coffee break

1035 - 1050  V. Laizé -Yeast aquaporins I
1050 - 1105  J. Carbrey - Yeast aquaporins II
1105 - 1120  M. Tamás - Function and regulation of the yeast MIP glycerol export channel Fps1p
1120 - 1135  A. Froger - Characterization of the first microbial mixed MIP: Gla of Lactococcus lactis
1135 - 1150  M. Hansen -Molecular cloning and functional expression of a water channel - aquaporin gene in Plasmodium falciparum

1150 - 1300  Lunch break

Sixth session: Regulation of aquaporins and other MIP channels

Chair: P. Deen, W. Rosenthal, and S. Sasaki

1300 - 1340  P. Deen (key lecture) - The trafficking of native and mutant mammalian MIP proteins
1340 - 1405  G. Valenti - Regulation of aquaporin-2 water channel trafficking by phosphorylation: lessons from transfected epithelial cells
1405 - 1430  D. Brown - cAMP-independent pathways of AQP2 membrane insertion
1430 - 1445  E. Klussmann - Identification of protein kinase A anchoring proteins potentially involved in AQP2 translocation and the role of the small GTPases of the Rho family in the translocation
1445 - 1500  J. Hoffert - Complex regulation of AQP5 expression by osmotic stress

1500 - 1530  Coffee

Seventh session: Clinical, developmental and applied aspects of MIP channel function

Chair: S. Nielsen, D. Marples and A. Aperia

1530 - 1555  R. W. Schrier -Pathophysiology of renal water and sodium metabolism in states of water retention
1555 - 1620  B. Rippe - Water transport in peritoneal dialysis
1620 - 1645  J. Kim - Ontogeny of renal aquaporins
1645 - 1700  J. Ågren - Perinatal increase in rat skin AQP 3 expression parallels maturation of skin barrier function and is influenced by antenatal corticosteroid treatment
1700 - 1715  S. Hirai - Deficiency of membrane localization of aquaporin 5 in lacrimal glands of Sjögren's syndrome patients and model mice
1715 - 1730  J. Han - Oxytocin: one of the factors for regulating AQP2 localization and urinary AQP2 excretion
1730 - 1750  J. Frøkiær - Dysregulation of renal aquaporins in postobstructive polyuria

1800 - 1900  Wine reception at Artisten

1900 -  Boat trip to Elfsborgs Fästning for the conference dinner

Wednesday 5 July

Young scientists event sponsored by the Commission of the European Union

Chair: J. Frøkiær, G. Valenti, S. Nielsen, C. Maurel, S. Hohmann, G. Calamita, and, H. Stahlberg

0815 - 0830  Introduction: a discussion forum for the reserach projects of young European scientists
0830 - 0845  N. Roudier - Studies of AQP3 oligomerisation by sucrose gradients and cryofracture
0845 - 0900  P. Francis - Missense mutations in the human MIP gene encoding the major intrinsic protein of the lens underlie dominant polymorphic lemellar cataratcs on 12q
0900 - 0915  V.Gresz - Identification and localisation of aquaporins expressed in human salivary glands
0915 - 0930  C. Cutler -The Expression of aquaporin homologues in fish
0930 - 0945  L. Virkki - Cloning and functional expresion of MIPfun, a major intrinsic protein homolog from killifish (Fundulus heteroclitus) lens
0945 - 1000  P. Savelkoul - Phosphorylation of ser256 is essential and seems sufficient for re-distribution of AQP2 from vesicles to the apical plasma membrane in MDCK cells

1000 - 1030   Coffee break

1030 - 1045  S. Combet - Renal aquaporin expression in aging rat: effect of food restriction
1045 - 1100  M. Carmosino -Functional expression of AQP4 in human gastric cell line
1100 - 1115  G. Cooper - Effect of expressing MIP-MOD and nodulin-26 on the NH3 permeability of Xenopus oocytes
1115 - 1130  D. Reisen - Dessiccation and osmotic stress trigger upregulation of tonoplast aquaporins
1130 - 1145  J. van Dongen - Functional analysis of four MIP-like proteins from the developing pea seed coat
1145 - 1200  F. Barrieu - Modulation of aquaporins genes expression in arabidopsis leads to altered membrane water permeability 

12 min talk and three min discussions

1200  Closing remarks - S. Nielsen and S. Hohmann

Conference ends