A major point of the project is testing biological concepts and mathematical models concerning the significance for the activation of signalling pathways in a cell a spatial redistribution of signalling proteins

"Statistical planning, analysis and bioinformatics tools in micro-array screenings, focusing on heavy metal stressed yeast cultures"

PhD in Mathematical Statistics, Dept. of Mathematical Statistics, Chalmers University of Technology

PhD student - ERIK KRISTIANSSON
erikri-8@student.luth.se

(starting date January 2003)
supervisor - OLLE NERMAN

PROJECT DESCRIPTION AND AIM

We intend to study the effects of heavy metal stress on yeast cells at a molecular level, using a functional genomics approach. The starting point will be to plan and analyse time series of cDNA array experiments with different stress levels of cadmium, arsenic and antimony. A sequential strategy with successive selections of interesting genes, using tailored chips and/or follow up with selective knock-out screening in new micro-array experiments and by phenotype analysis will be considered. The results will be compared between the different stresses and a second round of experiments will be performed on lead and mercury stressed cells.

We also anticipate use of bioinformatic based data-base screening for relevant facts on the extracted genes and if natural we will try to make joint analysis of the five stresses in order to make sense of our primary findings. We expect the project to give rise to a range of both mathematically, statistically and biologically relevant research problems of both straightforward and more difficult natures.

CONTACT INFORMATION

Supervisor

Professor Olle Nerman,

nerman@math.chalmers.se,

Tel: +46 (0)31/7723565.

COLLABORATING PAIR-PROJECT

"Heavy metals and the cell: identification of cellular targets and defence mechanisms"

PhD in Microbiology, Dept. of Cell and Molecular Biology, Göteborg University

PhD student - MICHAEL THORSEN (starting date November 2002) supervisors - STEFAN HOHMANN/MARKUS TAMAS



"Statistical planning, analysis and bioinformatics tools in micro-array screenings, focusing on heavy metal stressed yeast cultures"
PhD in Mathematical Statistics, Dept. of Mathematical Statistics, Chalmers University of Technology

	PhD student - ERIK KRISTIANSSON erikri-8@student.luth.se (starting date January 2003)		supervisor - OLLE NERMAN

		PROJECT DESCRIPTION AND AIM
We intend to study the effects of heavy metal stress on yeast cells at a molecular level, using a functional genomics approach. The starting point will be to plan and analyse time series of cDNA array experiments with different stress levels of cadmium, arsenic and antimony. A sequential strategy with successive selections of interesting genes, using tailored chips and/or follow up with selective knock-out screening in new micro-array experiments and by phenotype analysis will be considered. The results will be compared between the different stresses and a second round of experiments will be performed on lead and mercury stressed cells. We also anticipate use of bioinformatic based data-base screening for relevant facts on the extracted genes and if natural we will try to make joint analysis of the five stresses in order to make sense of our primary findings. We expect the project to give rise to a range of both mathematically, statistically and biologically relevant research problems of both straightforward and more difficult natures.
CONTACT INFORMATION Supervisor Professor Olle Nerman, nerman@math.chalmers.se, Tel: +46 (0)31/7723565.
COLLABORATING PAIR-PROJECT "Heavy metals and the cell: identification of cellular targets and defence mechanisms" PhD in Microbiology, Dept. of Cell and Molecular Biology, Göteborg University PhD student - MICHAEL THORSEN (starting date November 2002) supervisors - STEFAN HOHMANN/MARKUS TAMAS